This means that measurements made within QuPath and ImageJ should be comparable, because the images in both places have been calibrated accordingly. ImageJ can measure the total area and the damaged area. If you open the image properties in ImageJ ( Image ‣ Properties…), you see that the pixel size is 4 times larger than it is in QuPath. In the screenshot above, the downsampling value was set to 4. Therefore the area of the region selected to send, along with the downsampling, together control how large an image (in terms of pixels, and therefore memory) will be sent. downsample of 1) is quite likely to fail because of the amount of memory required by ImageJ to store the region. This is extremely important, since sending a very large region at the maximum high resolution (i.e. If it is 2, then the width and height will both be divided by 2 and the image rescaled accordingly.
If the downsampling factor is 1, then the full resolution image will be sent. Higher numbers indicate smaller images or, equivalently, lower magnification.
Must-read guidelines for effective acquisition and analysis of images.
IMAGEJ SOFTWARE TUTORIALS HOW TO
Our goal is to create a coherent, ordered set of tutorials, through which new people can proceed in order to understand how to use th. An introduction to the ImageJ and ImageJ2 applications. We have tutorials in several places throughout the SciJava GitHub organizations and on the ImageJ wiki. The downsampling effectively determines how much to scale (down) the region. The following tutorials are highly recommended for new users: 1.
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